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Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: Circular RNA circ_0081001 knockdown enhances methotrexate sensitivity in osteosarcoma cells by regulating miR-494-3p/TGM2 axis

Fig. 2

Downregulation of circ_0081001 inhibited MTX resistance by reducing cell viability and metastasis and promoting cell apoptosis in MTX-resistant OS cells. a, b The expression of circ_0081001 was analyzed in U2OS/R and HOS/R cells after transfection with sh-NC or sh-circ_00081001 using qRT-PCR analysis. c, d Cell viability and IC50 value were assessed by CCK-8 assay in U2OS/R and HOS/R cells transfected with sh-NC or sh-circ_00081001 and exposed to different concentrations of MTX. e Flow cytometry was performed to determine the cell apoptosis in U2OS/R and HOS/R cells after transfection with sh-NC or sh-circ_00081001. f, g Migration and invasion of U2OS/R and HOS/R cells were evaluated using the transwell assay after transfection with sh-NC or sh-circ_00081001. h, i WB analysis was conducted to examine the protein levels of cleaved-casp3, E-cadherin, and N-cadherin in U2OS/R and HOS/R cells after transfection with sh-NC or sh-circ_00081001. **P < 0.01

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X