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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Exosomal miR-186 derived from BMSCs promote osteogenesis through hippo signaling pathway in postmenopausal osteoporosis

Fig. 3

a The differentially expressed miRNAs were detected between BMSCs extracted in OVX rats (PMO) and BMSCs extracted in OVX rats + exosomes groups (PMO + Exos) using miRNA-seq. b q-PCR was performed to investigate the expression of miRNA between PMO and PMO + exosome group. c RNA in tibia of rats were extracted and the expression of miR-186 in tibia of rats were verified using q-PCR. d microT, miRanda, Target Scan, and PITA were used to predict the downstream targets of miR-186. e The binding sites between Mob1 and miR-186 were shown, and mutant or wild-type nucleotides were labeled in red. f The relationship between Mob1 and miR-186 was verified using the luciferase reporter assay. g RNA-pull down assays were conducted to further verify the interaction between Mob1 and miR-186. h q-PCR was performed to detect the expression of Mob1. i The expression of Mob1 was detected between the PMO and PMO + Exos groups using q-PCR

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X