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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Homoharringtonine inhibits fibroblasts proliferation, extracellular matrix production and reduces surgery-induced knee arthrofibrosis via PI3K/AKT/mTOR pathway-mediated apoptosis

Fig. 3

HHT inhibited fibroblast proliferation in vitro. a In vitro, cell viability was detected via CCK-8 assay. HHT induced dose- and time-dependent inhibitory effects of fibroblast proliferation. b Flow cytometry showed the cells were arrested at G1 phase and the cells in the S phase were significantly decreased. c In vitro, western blots shown that application of HHT could reduce the expression of Cyclin D1 and PCNA, which were known as cell proliferation protein markers. β-actin was used as a control. *P < 0.05 versus the control group. d EDU test showed that the percentage of EDU-positive cells decreased significantly with the increase of drug concentration

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