Table 1 Primer sequences for qRT-PCR
From: miR-1249-5p regulates the osteogenic differentiation of ADSCs by targeting PDX1
Gene | Forward primer 5′-3′ | Reverse primer 5′-3′ |
|---|---|---|
GAPDH | GACAGTCAGCCGCATCTTCT | GCGCCCAATACGACCAAATC |
U6 | GGCTTTTTGCGGTCTGG | GCTTCGGCAGCACATATACTAAAAT |
miR-1249-5p | ACACTCCAGCTGGGTTCACAGTGGCTAAG | AGGGCTTAGCTGCTTGTGAGCA |
OSX | AGACCTCCAGAGAGGAGAGAC | GGGGACTGGAGCCATAGTGA |
ALP | AATAGCCCTGGCAGATTCCC | CTCTCATGGTGTCTCGGTGG |
PDX1 | TGCCACTGTTGAGTGCAAGTC | ACCTGGAGAAGCCGAAGGTAA |