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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: miR-1249-5p regulates the osteogenic differentiation of ADSCs by targeting PDX1

Fig. 4

miR-1249-5p enhance the osteogenic differentiation of ADSCs. a PDX1, ALP, and OSX mRNA expression were detected by real-time RT-PCR. b PDX1, ALP, OSX, t-Akt, p-Akt, PI3K, and p-PI3K protein expressions were measured by western blot assay. c Alkaline phosphatase (ALP) and Alizarin Red staining (ARS) of miR-1249-5p mimic, inhibitor, and corresponding negative control after 7 days and 21 days differentiation respectively. *P < 0.05

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