Fig. 1

Identification of primary chondrocytes and OA cellular model. Expression levels of miR-129-3p and CPEB1 in cartilage were determined by qRT-PCR analysis (a, b); correlation analysis of miR-129-3p with CPEB1 (c); the morphology of chondrocytes (× 100), results of toluidine blue staining (purple, × 100), immunofluorescent staining of type II collagen (green, × 200), DAPI staining (blue, × 200), and the merge graph (× 200) (d). After TNF-α treatment, flow cytometry and Western blot were applied to detect chondrocyte apoptosis and cleaved caspase-3 expression, respectively (e, f). **P < 0.01. OA, osteoarthritis