Fig. 4

HCPT regulates autophagy through ATF6 pathway. a-b The expression levels of the autophagy markers LC3 were determined by western blot in ATF-6-knockdown and control group after treatment with or without 1 μg/mL HCPT, *P < 0.05. c ATF-6-knockdown and control group after treatment with or without 1 μg/mL HCPT were measured by LC3 immunofluorescence, the accumulation of LC3 punctate dots was decreased after silencing ATF6 gene. These experiments were performed in triplicate