Fig. 3

HCPT inhibits fibroblast proliferation by inducing autophagy. a-c The expression of Atg5, Beclin-1, LC3, p62 was determined by western blot analysis. β-actin was used as a control, *P < 0.05 versus 0 μg/mL. d TEM results showed that autophagic bodies appeared after HCPT treatment. Black arrows indicate autophagic bodies. e-f After treated with or without 3-MA, the fibroblasts were treated with 1 μg/mL HCPT, then detected by western blot analysis with antibodies specific for LC3, PCNA, and Cyclin D1, *P < 0.05. g After treated with or without 3-MA, the fibroblasts were treated with HCPT as described before and then cell viability was measured after 24 h. *P < 0.05 versus control group (0 μg/mL), #P < 0.05 versus HCPT treated only group (1 μg/mL). These experiments were performed in triplicate