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Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: The role of activating transcription factor 6 in hydroxycamptothecin-induced fibroblast autophagy and apoptosis

Fig. 2

HCPT induces apoptosis of fibroblasts through ATF6-mediated ERS. a-b The expression of CHOP, Bcl-2, and Bax was determined by western blot analysis in human fibroblast cells treated with 0, 0.05, 0.5, 0.1, and 4 μg/mL HCPT. *P < 0.05 vs 0 μ/ml group. β-actin was used as a control. c-d The expression of GRP-78 and ATF6 p50 were determined by western blot analysis, *P < 0.05 vs 0 μ/ml group. e Silenced ATF-6 p50 expression was verified by western blot analysis. f-g The expression levels of the ER stress markers GRP78 and CHOP and the downstream Bcl-2 and Bax were determined by western blot in ATF-6-knockdown and control group after treatment with or without 1 μg/mL HCPT, *P < 0.05. These experiments were performed in triplicate

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