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Fig. 5 | Journal of Orthopaedic Surgery and Research

Fig. 5

From: Cryopreserved, Thin, Laser-Etched Osteochondral Allograft maintains the functional components of articular cartilage after 2 years of storage

Fig. 5

BM-MSC co-culture results for migration and differentiation. May-Giemsa stain (a–c) showing the presence of BM-MSCs after 24 h of migration in a Transwell plate. Both fresh (b) and T-LE Allograft (c) co-cultures showed increased migration compared to BM-MSCs cultured alone (a). Alcian Blue stain (d, e) reveals the glycosaminoglycan (GAG) content present in the deposited ECM from the BM-MSCs co-culture. Both fresh and T-LE Allograft co-cultures induced chondrogenic differentiation resulting in an increase in GAG content in the cells compared to BM-MSCs cultured alone. Scale bars = 100 μm

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