Fig. 3

a Results from PrestoBlue metabolic assay. Graph represents the number of metabolically active cells throughout the duration of the explantation. A steady growth in viable cell count over time was observed for both fresh (14 days in media at 4 °C) and 2-year cryopreserved T-LE Allografts. Data presented as mean and standard error of the mean. b (× 20) Safranin-O stained outgrowth of cells from 12-week explanted cryopreserved T-LE Allograft into surrounding Fibrin glue. c–e Immunofluorescent staining results for explanted 2-year cryopreserved T-LE Allograft. Green fluorescence showing collagen II and red fluorescence showing aggrecan. The laser perorations (LE) can be seen in the T-LE Allograft, with cellular outgrowth (O) proliferating away from the graft edge (E), and into the surrounding fibrin glue. The T-LE Allograft maintains its extracellular matrix proteins, while metabolically active cells are secreting aggrecan into the surrounding fibrin glue. Scale bars = 100 μm