Fig. 4

HMGA2 inhibits the differentiation of MC3T3-E1 cells. miR-497-5p mimic + oe-HMGA2 or miR-497-5p mimic + oe-NC was transfected into MC3T3-E1 cells. a RT-qPCR measurement of HMGA2 expression in MC3T3-E1 cells after co-transfection. b MTT assessment of MC3T3-E1 cell viability. c Flow cytometric analysis for cell apoptosis. d RT-qPCR measurement of Bax, Bcl-2, OCN, and RUNX2 expression. e ALP staining for ALP activity. f ARS staining for mineralization. g Western blot detection of activation of the JNK pathway. Data are presented as the mean ± SD of three replicates each. The unpaired t test was used for the comparison between the two groups (a–c, e, f); the two-way ANOVA was applied for the comparison among multiple groups (d, g). *p < 0.05 vs. MC3T3-E1 cells transfected with NC mimic; ^@p < 0.05 vs. MC3T3-E1 cells transfected with miR-497-5p mimic + oe-NC