Fig. 3

HMGA2 is verified to be a target gene of miR-497-5p. a Binding regions between HMGA2 3′UTR and miR-497-5p sequence. b RT-qPCR measurement of HMGA2 mRNA expression in bone tissues of OP patients and controls. c RT-qPCR measurement of HMGA2 mRNA expression during osteogenic differentiation. d RT-qPCR measurement of HMGA2 mRNA expression in MC3T3-E1 cells after transfection. e Western blot measurement of HMGA2 protein expression in MC3T3-E1 cells after transfection. f Luciferase activity of the HMGA2-WT and HMGA2-MT after transfection. g RIP validation of the relationship between miR-497-5p and HMGA2. Data are presented as the mean ± SD of three replicates each. The unpaired t test was used for the comparison between the two groups (b–e); the two-way ANOVA was applied for the comparison among multiple groups (f, g). ^&&p < 0.01 vs. controls; ^%p < 0.05 vs. cells before induction; *p < 0.05 vs. MC3T3-E1 cells transfected with NC mimic; ^{^}p < 0.05 vs. cells treated with Anti-IgG