Table 1 The primers for each gene

GAPDH

forward: 5′-GAAGATGGTGATGGGATTTC-3′

reverse: 5′-GAAGGTGAAGGTCGGAGT-3′

U6

forward: 5′-CTCGCTTCGGCAGCACA-3′

reverse: 5′-AACGCTTCACGAATTTGCGT-3′

LEF-AS1

forward: 3′-TGT GAC TCC AGA GGC GGA AC-5′

reverse: 3′-AGG AGG CGG CAA GAA GAA GG-5′

miR-222-5p

forward: 5′-TGGCCTCGAGATGTGCTTCAG-3′

reverse: 5′-TCTCCTTGGCGGCCGCACTTCCTTC-3

RAMP3

forward: 5′-ACACCCGTGAGAGAGACTTG-3′

reverse: 5′-AAGTCAGTCGGGAAGGAAGG-3′