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Fig. 1 | Journal of Orthopaedic Surgery and Research

Fig. 1

From: Knockdown of long non-coding RNA LEF1-AS1 attenuates apoptosis and inflammatory injury of microglia cells following spinal cord injury

Fig. 1

LncRNA LEF-AS1 was upregulated in the SCI model in vivo and in vitro. a BBB scores were measured in sham and SCI groups. b Relative expression of lncRNA LEF-AS2 in sham and SCI groups. c Relative expression of lncRNA LEF-AS2 in control and LPS-treated microglia cells. d Cell viability in control and LPS-treated microglia cells measured by CCK-8 assay. e Apoptosis ratio in control and LPS-treated microglia cells measured by Annexin V/FITC apoptosis assay. f Western blot assay to measure the protein expression of apoptotic markers (Bax, Bcl-2, and cleaved-caspase-3). g Inflammatory cytokines (TNF-α and IL-6) expression levels in control and LPS-treated microglia cells (P < 0.05)

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