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Fig. 5 | Journal of Orthopaedic Surgery and Research

Fig. 5

From: Autophagy in spinal ligament fibroblasts: evidence and possible implications for ossification of the posterior longitudinal ligament

Fig. 5

Role of Beclin1 in osteogenic differentiation of ligament fibroblasts. RT-qPCR assay for Beclin1 (a), OCN (b), COL 1 (c), and ALP (d) in OPLL and non-OPLL ligament fibroblasts transfected with siBeclin1 or siControl for 72 h. In OPLL ligament fibroblasts, after knockdown by siBeclin1, the expression of Beclin1, OCN, COL 1, and ALP genes were reduced by 63.2% (P < 0.01), 52% (P < 0.01), and 53.2% (P < 0.01), respectively. However, no significant difference was observed between the siBeclin1, siControl, and control groups in non-OPLL ligament fibroblasts (n = 12 for each group). OPLL, ossification of the posterior longitudinal ligament; RT-qPCR, reverse transcription-quantitative PCR; ALP, alkaline phosphatase, biomineralization associated; COL 1, collagen type 1; OCN, osteocalcin; si, small interfering

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X