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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: Autophagy in spinal ligament fibroblasts: evidence and possible implications for ossification of the posterior longitudinal ligament

Fig. 4

Representative fluorescence images showing immunofluorescence assay for LC3 protein (green), Beclin1 (red), and merged images under the two different conditions. Scale bar, 40 μm. An increase of Beclin1 and LC3 proteins were observed in the cytoplasm of the OPLL-derived ligament fibroblasts compared with the non-OPLL-deprived ligament fibroblasts (n = 12 for each group). *P < 0.05, **P < 0.01 OPLL vs. non-OPLL. OPLL, ossification of the posterior longitudinal ligament; LC3, microtubule-associated protein 1 light chain 3

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