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Fig. 6 | Journal of Orthopaedic Surgery and Research

Fig. 6

From: Identification of key pathways and hub genes in the myogenic differentiation of pluripotent stem cell: a bioinformatics and experimental study

Fig. 6

Experimental certification of the hub genes and key pathways. a Cellular morphology of passage-3 ADSCs. b MTT assessment showing the effect of different concentrations of 5-Aza on the viability of ADSCs at passage 3 after 1, 3, 5, 7, and 9 days of exposure. The x-axis is the time (days), and the y-axis is the cell viability value. c Flow cytometry analysis results and expression of cell surface CD markers of ADSCs at passage 3. The x-axis is the fluorescence intensity, and the y-axis is the cell number. e Immunofluorescence analysis of ADSCs. The results represent sarcomeric-α-actinin expression in ADSCs exposed to three myogenic concentration protocols. e Myogenic differentiation rates are represented by the percent expression of actin as measured by immunohistochemistry. f MyoD (RT-PCR) mRNA expression levels

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X