Fig. 4

Silencing of RGMA promotes the neurological recovery in rats following SCI. a Expression of miR-29a, miR-199B, and mRNA of RGMA in gray matter determined by RT-qPCR. b Protein level of RGMA in gray matter evaluated by western blot analysis. c NF-200 expression in the gray matter 2–3 mm posterior to the injury sites using immunofluorescence staining. d Protein levels of NF-200 and synaptophysin in the gray matter 2–3 mm posterior to the injury sites detected by western blot analysis. N = 8. Data were presented as mean ± SD from at least three independent experiments. Data were compared by one-way or two-way ANOVA. *p < 0.05 vs sh-NC; ^#p < 0.05 vs sh-RGMA + NC inhibitor