Fig. 3

miR-29a and miR-199B negatively regulate RGMA and inactivate the STAT3 signaling pathway. a Protein levels of RGMA and STAT3 as well as phosphorylation of STAT3 in tissues in gray matter of SCI model rats determined by western blot analysis. b, c Putative binding sites between miR-29a (b) and miR-199B (c) and RGMA predicted on StarBase (http://starbase.sysu.edu.cn/). d, e Binding relationships between miR-29a (d) and miR-199B (e) and RGMA validated through luciferase assays. f Protein levels of RGMA and STAT3 as well as phosphorylation of STAT3 in tissues in gray matter of SCI model rats after miR-29a/199B transfection determined by western blot analysis. N = 8. Data were presented as mean ± SD from at least three independent experiments. Data were compared by one-way or two-way ANOVA. *p < 0.05 vs sham group or NC mimic