Fig. 4

Effects of ODNs on osteogenic differentiation gene expression. a Quantitative real-time PCR analysis of SP7 and OCN mRNA expression of MC3T3 cells treated with selected CpG ODNs for 1 day, 3 days and 5 days. n = 3. b Western blot analysis of SP7 and OCN protein levels of MC3T3 cells treated with selected CpG ODNs for 3 days. n = 3. Data were expressed as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with the control group and ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 compared with the ODN 2006 group by paired t test