Fig. 3

Effects of ODNs on osteogenic differentiation. a ALP activity assay and b ALP staining assay of MC3T3 cells treated by 12 ODNs for 24 h, 48 h, 72 h, and 7 days, respectively. 1, FC003; 2, SAT05f; 3, SAT05d; 4, MS19; 5, BW001; 6, FC001; 7, FC002; 8, BW006; 9, YW002; 10, YW001; 11, FC004; 12, MT01. n = 3. Data were expressed as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with the control group and ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 compared with the ODN 2006 group by paired t test