Table 1 Primers sequence of target gene for RT-PCR
Genes | Primer sequence | Size (bp) |
|---|---|---|
HIF-1α | Forward:5′- GTCGCTTCGGCCAGTGTG-3′ | 155 |
|  | Reverse:5′-GGAAAGGCAAGTCCAGAGGTG-3′ |  |
Col II | Forward:5′-TCCCAGAACATCACCTACCA-3′ | 154 |
|  | Reverse:5′-CATCCTGCAGCACGGTATAG-3′ |  |
Aggrecan | Forward:5′-GCTGCTACGGAGACAAGGAT-3′ | 107 |
|  | Reverse:5′-CTCACCCTCCATCTCCTCTG-3′ |  |
GAPDH | Forward:5′-CCCTCAATGACCACTTTGTG-3′ | 117 |
|  | Reverse:5′-GGTTTGAGGGCTCTTACTCCT-3′ |  |