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Table 1 Primers sequence of target gene for RT-PCR

From: A possible injectable tissue engineered nucleus pulposus constructed with platelet-rich plasma and ADSCs in vitro

Genes Primer sequence Size (bp)
HIF-1α Forward:5′- GTCGCTTCGGCCAGTGTG-3′ 155
  Reverse:5′-GGAAAGGCAAGTCCAGAGGTG-3′  
Col II Forward:5′-TCCCAGAACATCACCTACCA-3′ 154
  Reverse:5′-CATCCTGCAGCACGGTATAG-3′  
Aggrecan Forward:5′-GCTGCTACGGAGACAAGGAT-3′ 107
  Reverse:5′-CTCACCCTCCATCTCCTCTG-3′  
GAPDH Forward:5′-CCCTCAATGACCACTTTGTG-3′ 117
  Reverse:5′-GGTTTGAGGGCTCTTACTCCT-3′