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Table 1 Sequences of primers for quantitative real-time PCR

From: Apolipoprotein D alleviates glucocorticoid-induced osteogenesis suppression in bone marrow mesenchymal stem cells via the PI3K/Akt pathway

Gene

Forward primer (5′-3′)

Reverse primer (3′-5′)

GAPDH

TTCTTTTGCGTCGCCAGCCGA

GTCACCACCCGCCCAATACGA

RUNX2

GGGTAAGACTGGT-CATAGGACC

CCCAGT-ATGAGAGTAGGTGTCC

ALP

ACCACCACGAGAGTGAACCA

CGTTGTCTGAGTACCAGTCCC

Osterix

AGGAGGCACAAAGAAGCCATAC

AGGGAAGGGTGGGTAGTCATT

Apod

TTAACCTCACAGAGCCTGCC-3

GAGTCCACTGTTTCTGGAGGG

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X