Fig. 4

miR-1323/BMP4 and miR-1323/SMAD4 axes regulate osteogenic differentiation by modulating the nuclear translocation of TAZ. a-d Human mesenchymal stromal cells were placed in osteogenic differentiation medium and infected with LV-SMAD4 or LV-BMP4 and co-transfected with a miR-1323 mimics. After 7 days of culture, TAZ levels were measured in the cytoplasm and nucleus and normalized to histone H3 and β-actin. *p < 0.05; **p < 0.01 [vs. NC mimics+LV-NC]; ^†P < 0.05, ^††P < 0.01 [vs. NC mimics+LV-BMP4 or NC mimics+LV-SMAD4]. e Cells were treated with vehicle (PBS) or verteporfin and the protein expression of ALP, Col I, and RUNX2 was determined. f The activity of ALP was measured. *p < 0.05; **p < 0.01 [vs. PBS]. g Mineralization degree determined by staining with Alizarin Red and ALP. Scale bars = 100 μm. Data presented as means ± SEMs. All in vitro experiments: 3 biological replicates × 3 technical replicates