Fig. 2

miR-1323 modulates mesenchymal stromal cell differentiation. Mesenchymal stromal cells were placed in osteogenic differentiation medium and transfected with miR-1323 mimics or inhibitors. a After 7 days of culture, qRT-PCR was used to confirm miR-1323 levels. b, c ALP, Col I, and RUNX2 protein expression was measured via Western blot. d The activity of ALP was measured. e Mineralization degree determined by staining with Alizarin Red and ALP. Scale bars = 100 μm. *p < 0.05, **p < 0.01 [miR-1323 inhib vs. NC inhib; miR-1323 mimics vs. NC mimics]. Data presented as means ± SEMs. All in vitro experiments: 3 biological replicates × 3 technical replicates