Skip to main content
Fig. 1 | Journal of Orthopaedic Surgery and Research

Fig. 1

From: miR-1323 suppresses bone mesenchymal stromal cell osteogenesis and fracture healing via inhibiting BMP4/SMAD4 signaling

Fig. 1

Downregulation of BMP4/SMAD4 signaling and osteogenesis-related markers in atrophic non-union fractures. a Histopathological analysis of atrophic nonunion (ANU) (n = 5) and standard healing fracture (SHF) specimens (n = 5) using H&E and Alcian Blue staining. IHC analysis of BMP4 and SMAD4 protein expression in ANU and SHF specimens. Scale bars = 100 μm. b qRT-PCR analysis of miR-1323 expression in ANU and SHF specimens. miR-1323 expression normalized to the SHF median value. c Representative immunoblots and d quantitation of BMP4, SMAD4, ALP, Col I, and RUNX2 protein expression in ANU and SHF specimens. Protein expression normalized to the SHF median values. e ALP activity in ANU and SHF specimens. ALP activity reported as the optical density (OD) 405 nm value per mg protein. f, g Pearson correlation analyses of (f) miR-1323 expression and BMP4 expression as well as (g) miR-1323 expression and SMAD4 expression in ANU specimens. *p < 0.05, **p < 0.01 [SHF vs. ANU]. b, d, e Data presented as medians ± interquartile ranges (boxes) and absolute ranges (whiskers)

Back to article page