Fig. 4

URG4 regulates osteosarcoma cell growth by activating the GSK3β/β-catenin/cyclin D1 signaling pathway. a URG4-related genes enriched in the top 10 significant signaling pathway based on KEGG. b The protein level of GSK3β, p-GSK3β, nuclear β-catenin, and cyclin D1 in the HOS cell transfected with siURG4 was revealed by the western blot assay. c The protein level of GSK3β, p-GSK3β, nuclear β-catenin, and cyclin D1 in the MG63 cell transfected with siURG4 was revealed by the western blot assay. d The positive expression of the nuclear β-catenin protein in osteosarcoma cells was measured by the immunohistochemistry assay. e A model depicted URG4 promotes osteosarcoma progression via the GSK3β/β-catenin/cyclin D1 pathway. URG4: upregulated gene 4; NC: negative control. *p < 0.05 vs the NC