Table 1 Primers used for reverse transcription and PCR in this study
Gene | Primer sequence (5′–3′) |
|---|---|
RUNX2 | F: CTCTTCCCAAAGCCAGAGTC |
R:CAGCGTCAACACCATCATTC | |
OSX | F: GAGGAAGAAGCCCATTCACA |
R:GCAGGCAGGTGAACTTCTTC | |
ALP | F: AACCCAGACACAAGCATTCC |
R:GCCTTTGAGGTTTTGGGTCA | |
OPN | F:GACGATGATGACGATGG |
R:CCTCAGTCCATAAGCCAAGC | |
GADPH | F:AAGGTCATCCCAGAGCTGAA |
R:AGGAGACAACCTGGTCCTCA | |
U6 | F:ATTGGAACGATACAGAGAAGATT |
R:AGGAACGCTTCACGAATTTG | |
miR-505 | F:GCGGGAGCCAGGAAGTAT |
R:CAGTGCGTGTCGTGGAGT |