Table 1 Primers used in this study*
Gene | Forward Primer (5'->3') | Reverse Primer (5'->3') | |
|---|---|---|---|
Adipogenic | PPARγ | GAGGCAGCAGAGGTTAACAGA | CACCGAGGCGTAAAGTACCA |
LPL | CCGCCGACCAAAGAAGAGAT | TAGCCACGGACTCTGCTACT | |
FABP4 | TGGGCCAGGAATTTGACGAA | CACATGTACCAGGACACCCC | |
Osteogenic | RUNX2 | CCTACCTGAGCCAGATGACG | ATGCTGGGTGGCCTGAAAT |
ALP | GAATCTTCCCCAAGGGCCAA | CAGAATGTTCCACGGAGGCT | |
BGLAP | TCCTTTGGGGTTTGGCCTAC | CCAGCCTCCAGCACTGTTTA | |
Chondrogenic | SOX9 | AGGAAGTCGGTGAAGAACGG | AAGTCGATAGGGGGCTGTCT |
COL2 | GCTCCTGCCGTTTCGCTG | ATTATACCTCTGCCCATCCTGC | |
ACAN | CTTCCGCTGGTCAGATGGAC | CGTTTGTAGGTGGTGGCTGT | |
Tenogenic | SCX | ACAGATCTGCACCTTCTGCC | GCCACCTCCTAACTGCGAAT |
TNMD | TCCCTCAGGCTCTGGTATGA | AGGACTGAGAGACCACTGCT | |
DCN | TGCCAAAGGATCTTCCCCCT | AGGTGTAAATGCTCCAGGACT | |
COL1A1 | AGTGGTTTGGATGGTGCCAA | GCACCATCATTTCCACGAGC | |
COL3A1 | TGGAGGATGGTTGCACGAAA | ACAGCCTTGCGTGTTCGATA | |
TNC | ATGGGCAGACGCACCATTAG | TGTGCATCGACCTTCACAAGA | |
Internal control | 18S rRNA | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG |