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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: The JNK pathway represents a novel target in the treatment of rheumatoid arthritis through the suppression of MMP-3

Fig. 3

JNK inhibitor (SP600125) has anti-oxidative and anti-inflammatory effects. a(1) An illustrative Western blot picture of phosphorylated JNK (54 and 46 kD) in MH7A cells following SP600125 treatment (15 and 30 μM) for 3 h. a(2, 3) Normalized intensities were graphed (N = 3). Normalization was performed with phosphorylated JNK (54 and 46 kD) of untreated MH7A cell (3-h incubation). b(1, 2) ROS formation assay in MH7A cells by H2O2 treatment (100 μM) for 1 h following SP600125 treatment (15 and 30 μM) for 3 and 24 h was evaluated by Muse Cell Analyzer. c This graph showed the mean concentrations of IL-6 in supernatant of MH7A cells determined by chemiluminescent enzyme immunoassay (CLEIA) following 24 h after the H2O2 treatment (100 μM) and SP600125 (30 μM). All values are expressed as the mean ± SEM (N = 3). The significance levels are shown as *P value < .05, **P value < .01, ***P value < .001, ****P value < .0001

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