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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: Artesunate prevents knee intraarticular adhesion via PRKR-like ER kinase (PERK) signal pathway

Fig. 4

ART induced ER stress in the fibroblasts. a, b The expression of CHOP, Bcl-2, and Bax in cells that had been treated with 0, 5 μM, 10 μM, 20 μM ART overnight was determined by western blot analysis. GAPDH was included as a control. cf The dose- and time-dependent effects of ART on the expression of proteins associated with the PERK signaling pathway, including p-PERK, PERK, p-eIF2α, and eIF2α, were determined by western blot analysis. GAPDH was used as a control. g, h After treated with or without GSK2606414, the fibroblasts were treated with 10 μM ART for 24 h, the detected by Western blotting with antibodies specific for GRP78, CHOP, Bax, Bcl-2, and GAPDH. i After treated with or without GSK2606414, the fibroblasts were treated with ART as described before and then cell viability was measured. *P < 0.05 versus control group

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