Fig. 2

GW4064 treatment indirectly regulated cyclin G1 (CCNG1) expression via microRNA-23b-3p. CCNG1 was identified as a promising target of miR-23b-3p. Considering the positive correlation between farnesoid X receptor (FXR) and miR-23b-3p, we further assessed the correlation between FXR and CCNG1 expression. a The changes of CCNG1 mRNA and protein levels were measured by quantitative PCR (qPCR) and Western blot under the effects of increased concentrations of GW4064. b The transfection efficiency of FXR siRNA (siFXR) was measured by qPCR and Western blot. c The combined effect of siFXR and GW4064 on the expression of miR-23b-3p was examined by qPCR. d The combined effect of siFXR and GW4064 on CCNG1 expressions was measured by qPCR and Western blot. e We further transfected miR-23b-3p inhibitor and inhibitor control into MG-63 cells. The single and combined effects of inhibiting miR-23b-3p and GW4064 effects on CCNG1 were detected by qPCR and Western blot. f The changes of MG-63 cell viability were determined by Cell Counting Kit-8 (CCK-8) assay. Each value represents mean ± SEM (n = 3). GAPDH and U6 served as internal controls for cellular genes and miR-23b-3p, respectively. **p < 0.01 vs. blank (siNC or IC) group; ^##p < 0.01 vs. siNC + GW4064 group; ^p < 0.05, ^^p < 0.01 vs. I group