Fig. 1

Expression of Cadherin-11 and P-Akt in OA FLS induced by TNF-α with or without PI3K inhibitor LY294002. a Real-time PCR showed that cadherin-11 mRNA increases with the stimulation of TNF-α in OA FLS (p < 0.05), but PI3K inhibitor LY294002 had no significant inhibitory effect (p > 0.05). b Western blot showed that expression of Cadherin-11 and phosphorylation of Akt (P-Akt) increased with the stimulation of TNF-α in OA FLS (p < 0.001), while PI3K inhibitor LY294002 had significant inhibitory effect (p < 0.001). c Quantification analysis of expression of Cadherin-11 protein. The amount of each protein was determined densitometrically and normalized to β-actin. d Quantification analysis of P-Akt. The amount of each protein was determined densitometrically and normalized to total Akt. The experiment was performed three times. Values were means ± SD, *p < 0.05, **p < 0.01, ***p < 0.001