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Fig. 1 | Journal of Orthopaedic Surgery and Research

Fig. 1

From: Emodin promotes fibroblast apoptosis and prevents epidural fibrosis through PERK pathway in rats

Fig. 1

a After fibroblast treated with various concentrations of EMO for 24 h, the CCK-8 assays showed that EMO inhibited the cell viability in a concentration dependent. b Following the 10 μg/ml EMO-treated fibroblasts, the CCK-8 assays showed that EMO could inhibit the cell viability in a time-dependent manner. c After treatment of fibroblasts with EMO of 10 μg/ml for 24 h. TUNEL staining was performed to detect the effect of EMO in promoting apoptosis. All nuclei of fibroblasts were stained blue by DAPI, and the nuclei of TUNEL-positive fibroblasts were red. d We picked three fields to calculate the percentage of apoptotic fibroblasts in each group. *P < 0.05 versus the control group. e Western blot analysis showed that the expression of apoptosis-related proteins Bcl-2, Bax, and cleaved PARP changed with the increasing of EMO concentration. GAPDH was used as a control. f Annexin V-FITC/PI double labeling was performed to detect fibroblast apoptosis after 10 μg/ml EMO treated for 24 h. g The histogram presented the apoptotic rate after the treatment with EMO by statistical method. The result was repeated for three times. *P < 0.05 versus the control group

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