Table 1 Sequences of primers used for qRT-PCR analysis
Primers | Sequence 5′-3′ (forward/reverse) | Reference |
|---|---|---|
RT-qPCR | ||
icaA | 5′-GATTATGTAATGTGCTTGGA-3′/ 5′-ACTACTGCTGCGTTAATAAT-3′ | This study |
icaD | 5′-ATGGTCAAGCCCAGACAGAG-3′/ 5′-CGTGTTTTCAACATTTAATGCAA-3′ | This study |
icaB | 5′-CACATACCCACGATTTGCAT-3′/ 5′-TCGGAGTGACTGCTTTTTCC-3′ | This study |
yycF | 5′-TGGCGAAAGAAGACATCA-3′/ 5′-AACCCGTTACAAATCCTG-3′ | This study |
yycG | 5′-CGGGGCGTTCAAAAGACTTT-3′/ 5′-TCTGAACCTTTGAACACACGT-3′ | This study |
16S rRNA | 5′-GTAGGTGGCAAGCGTTATCC-3′/ 5′-CGCACATCAGCGTCAACA-3′ | This study |