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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: Icariin alleviates osteoarthritis by inhibiting NLRP3-mediated pyroptosis

Fig. 4

ICA suppressed chondrocytes injury and pyroptosis through NLRP3. a The mRNA level of NLRP3 was validated by qRT-PCR in chondrocytes. b The protein level was assessed by Western blotting in chondrocytes. GAPDH was used as internal control. c Leakage of LDH was assessed by ELISA assay in chondrocytes. The release level of IL-1β (d) and IL-18 (e) were analyzed by ELISA assay in chondrocytes. f The protein level of pyroptosis-related protein was analyzed by Western blotting in rat chondrocytes. g Quantitative analysis of protein band gray in e.GAPDH was used as internal control. All the results were shown as mean ± SD (n = 3), which were three separate experiments performed in triplicate. *p < 0.05 and **p < 0.01

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