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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Icariin alleviates osteoarthritis by inhibiting NLRP3-mediated pyroptosis

Fig. 3

ICA exerted negative effects on LPS-induced NLRP3 expression and pyroptosis. a Immunofluorescent was used to detect NLRP3 in chondrocytes treated with LPS or ICA. b Flow cytometry analysis was used to measure caspase-1 activity in chondrocytes treated with LPS or ICA. c Quantitative statistics of doublepositive for caspase-1 and PI stain. d The protein level of pyroptosis-related protein was analyzed by Western blotting in chondrocytes treated with LPS or ICA. e Quantitative analysis of protein band gray in d. GAPDH was used as internal control. All the results were shown as mean ± SD (n = 3), which were three separate experiments performed in triplicate. *p < 0.05 and **p < 0.01

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