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Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: Impairment of circulating endothelial progenitor cells (EPCs) in patients with glucocorticoid-induced avascular necrosis of the femoral head and changes of EPCs after glucocorticoid treatment in vitro

Fig. 2

EPCs from glucocorticoid-induced ANFH patients had decreased cell number and functions. a, b GC-induced ANFH patients have less circulating EPCs in peripheral blood. a Representative diagrams of flow cytometry analysis of CD34+CD133+ cells from healthy controls (left panel) and patients (right panel). b Quantification of EPC numbers from the two groups. c, d Chemotaxis assay showed EPCs from GC-induced ANFH patients had impaired function of migration. c Representative images of cells incubated in Zigmond chamber for 0 and 6 h as indicated. Cells’ mobilization towards SDF1 and lamellipodia formation was observed in the control group (HC, upper panels) but not in patient group (PT, lower panels). Bar = 10 μm. d Average cell moving distance in the two groups after a 24-h incubation. e, f Tube formation assay showed EPCs from GC-induced ANFH patients had impaired function of angiogenesis. e Representative images of tube formation. Left panels, images with no highlights. Right panels, images with highlights outlining the tubules. f The average diameter of tubules formed by cells in each group. HC healthy controls, PT patients; *p < 0.05, **p < 0.01, ***p < 0.001

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X