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Fig. 5 | Journal of Orthopaedic Surgery and Research

Fig. 5

From: TGF-β1 reduces the oxidative stress-induced autophagy and apoptosis in rat annulus fibrosus cells through the ERK signaling pathway

Fig. 5

TGF-β1 inhibits the H2O2-induced apoptosis in annulus fibrosus cells. a Western blot to show the expression of Bax, Bcl-2, and cytoplasmic/mitochondrial cyt-C. b Mitochondrial membrane potential. c Western blot analysis to indicate the expressions of caspase-9 and caspase-3. d Flow cytometry analysis to investigate cell apoptosis. TGF-β1 transforming growth factor-β1, H2O2 hydrogen peroxide, n.s not significant; *P < 0.05; **P < 0.001

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