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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: TGF-β1 reduces the oxidative stress-induced autophagy and apoptosis in rat annulus fibrosus cells through the ERK signaling pathway

Fig. 3

H2O2-induced apoptosis in annulus fibrosus cells. a Western blot analysis to indicate the expressions of cleaved caspase-3 and PARP. b The enzymatic activity test for caspase-9, caspase-3, and caspase-8. c Western blot to show the expression of caspase-12, Bax, Bcl-2, and cytoplasmic/mitochondrial cyt-C. d Mitochondrial membrane potential. n.s not significant; *P < 0.05; **P < 0.001

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