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Fig. 2 | Journal of Orthopaedic Surgery and Research

Fig. 2

From: TGF-β1 reduces the oxidative stress-induced autophagy and apoptosis in rat annulus fibrosus cells through the ERK signaling pathway

Fig. 2

TGF-β1 attenuates the H2O2-induced autophagy in annulus fibrosus cells. a Western blot analysis to indicate the expression of autophagy-related proteins (Beclin-1 LC3 II/I, and p62) in annulus fibrosus cells after treatment with 100 μmol/L H2O2. b Western blot analysis to indicate the expression of autophagy-related proteins (Beclin-1, LC3 II/I, and p62) in annulus fibrosus cells after treatment with 100 μmol/L H2O2 and TGF-β1. c Green fluorescence to observe the GFP-LC3 in autophagosomes after treatment with TGF-β1 and autophagy inhibitor Baf. TGF-β1, transforming growth factor-β1. H2O2 hydrogen peroxide, Baf Bafilomycin, n.s not significant; *P < 0.05; **P < 0.001

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X