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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: BMSC affinity peptide-functionalized β-tricalcium phosphate scaffolds promoting repair of osteonecrosis of the femoral head

Fig. 3

The rabbit BMSCs were incubated with FITC-labeled LSP, DPI, or RGD and analyzed by FCM. a The peak of the experimental group (DPI) and positive control group (RGD) shifted to the right markedly compared with the negative control group (LSP). b The average fluorescence intensity for the cells incubated with FITC-DPI was higher than that of FITC-LSP (n = 3; P = 2.733 × 10−8). c The cells incubated with FITC-LSP, FITC-DPI, or FITC-RGD were observed under a fluorescence microscope. Strong FITC signals were observed in cells incubated with FITC-DPI and FITC-RGD, whereas no FITC signals were observed in cells incubated with FITC-LSP. The nuclei were stained with DAPI. The cytoskeleton was stained with rhodamine phalloidin; scale bar, 100 μm

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