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Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Autologous cell-coated particles for the treatment of segmental bone defects—a new cell therapy approach

Fig. 3

Osteogenic gene expression after osteogenic induction for 2 days. a qPCR analysis of early osteogenic markers ALP, SP7 (Osterix), and DLX5 gene expression at day 2 after osteogenic induction. b Time course of the late osteogenic markers SPP1 and IBSP. Values were normalized to day 0. Real-time analysis was done using ΔΔCt method and shown as fold change vs. control group and normalized to the RPLP0 housekeeping gene. Mean ± SEM (n = 6). c FACS analysis revealed that ~ 50% of the cells express SP7 protein after 2 days of osteogenic induction in the 3D system

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X