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Fig. 4 | Journal of Orthopaedic Surgery and Research

Fig. 4

From: Combined low-dose LiCl and LY294002 for the treatment of osteoporosis in ovariectomized rats

Fig. 4

Effects of LiCl, LY294002, and LiCl + LY294002 on bone resorption and TRAP activity in osteoclasts. Control: cells were cultured in α-MEM. LiCl: cells were cultured in α-MEM with LiCl (10 mM). LY294002: cells were cultured in α-MEM with LY294002 (10 μM). LiCl + LY294002: cells were cultured in α-MEM with LiCl (5 mM) + LY294002 (5 μM). a Images showing osteoclasts in bone slices after 14 days of culture; scale bar = 200 μM. b Statistics of the pit area fragment (**P < 0.01; n = 3). c TRAP activity in osteoblasts was determined using a kit. The results are expressed as specific activity (king unit/mgprot) of osteoblasts cultured for 21 days (**P < 0.01; n = 3)

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