Fig. 3
From: Combined low-dose LiCl and LY294002 for the treatment of osteoporosis in ovariectomized rats
Mineralized nodules as visualized by Alizarin red staining and ALP activity in osteoblasts. a A total of 1.1 × 104 osteoblasts per square centimeter were cultured in a 35-mm dish with either α-MEM, α-MEM with LiCl (10 mM), α-MEM with LY294002 (10 μM), or α-MEM with LiCl (5 mM) + LY294002 (5 μM) for 21 days (n = 3). b Quantitative results from Alizarin red staining of rat osteoblasts in each group. The counts of osteoblasts treated with LiCl + Ly294002 were significantly increased (**P < 0.01; n = 3). c ALP activity in osteoblasts was determined by a kit. The results are expressed as specific activity (king unit/mgprot) of osteoblasts cultured for 21 days (**P < 0.01; n = 3)