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Fig. 1 | Journal of Orthopaedic Surgery and Research

Fig. 1

From: Combined low-dose LiCl and LY294002 for the treatment of osteoporosis in ovariectomized rats

Fig. 1

Western blot results for AKT/GSK3β/β-catenin/NFATC1 signaling in osteoblasts. a The results obtained from Western blot analyses indicate that the protein expression levels of p-AKT, GSK-3β, p-GSK-3β, p-β-catenin, and NFATC1 were significantly decreased and that of p-AKT was significantly increased in the samples treated with LiCl [cells cultured in α-MEM with LiCl (10 mM)], LY294002 [cells cultured in α-MEM with LY294002 (10 μM)], and LiCl + LY294002 [cells cultured in α-MEM with LiCl (5 mM) + LY294002 (5 μM)] compared with the samples treated with vehicle (cells cultured in α-MEM). b At 30 min, the ratio of p-AKT/AKT in cells cultured in LY294002 and LiCl + LY294002 was found to have significantly increased (**P < 0.01; n = 3). c At 30 min, the ratio of p-GSK3β/GSK3β in cells cultured in LiCl, LY294002, and LiCl + LY294002 was found to have significantly increased (**P < 0.01; n = 3). d At 30 min, the ratio of p-β-catenin/β-catenin in cells cultured in LiCl, LY294002, and LiCl + LY294002 was found to have significantly decreased (**P < 0.01; n = 3). e At 30 min, the ratio of NFATC1/β-actin in cells cultured in LiCl, LY294002, and LiCl + LY294002 was found to have significantly decreased (**P < 0.01; n = 3)

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