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Fig. 1 | Journal of Orthopaedic Surgery and Research

Fig. 1

From: Combined ascorbic acid and T3 produce better healing compared to bone marrow mesenchymal stem cells in an Achilles tendon injury rat model: a proof of concept study

Fig. 1

rBMSC characterization. An example of rBMSCs phenotype and functional analysis. a Flow cytometry analysis of MSCs for surface markers (CD45+ isotype control, CD90+ isotype control, negative control) showed that isolated bone marrow-derived cells expressed CD90, but not the hematopoietic marker CD45, confirming that only mesenchymal cells had been isolated (CD45−CD90+ cells population). b–d In vitro differentiation of mesenchymal cells: the cells were incubated in lineage-specific induction media and then analyzed by histochemical and cytological staining for b adipocytes (Oil Red-O staining), c osteoblasts (alizarin red staining), and d chondrocytes (Alcian Blue staining)

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Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X