Fig. 4

The recruitment of Brd3 and Brd4 to the chromatin responsible for IL-1β- or TNF-α-induced transcription. a–d Human chondrocytes were pretreated with or without I-BET151 (1 μM), followed by addition of vehicle, IL-1β (10 ng/ml) or TNF-α (10 ng/ml) for 6 h, and ChIP assays were performed for Brd3. e–h Human chondrocytes were pretreated with or without I-BET151 (1 μM), followed by addition of vehicle, IL-1β (10 ng/ml) or TNF-α (10 ng/ml) for 6 h, and ChIP assays were performed for Brd4. The quantitative analysis of targeted promoter regions was determined by real-time PCR using specific primers for MMP1, MMP3, MMP13, and ADAMTS4. Relative fold-change values were calculated in comparison with the vehicle control that was set to 1 (n = 2)