Skip to main content
Fig. 3 | Journal of Orthopaedic Surgery and Research

Fig. 3

From: Recruitment of Brd3 and Brd4 to acetylated chromatin is essential for proinflammatory cytokine-induced matrix-degrading enzyme expression

Fig. 3

The effect of specific siRNAs on regulation of matrix-degrading enzyme gene transcription in SW1353 cells. SW1353 cells were treated with scrambled siRNA or specific siRNA targeting BRD2, BRD3, and BRD4 for 48 h and then treated with or without IL-1β (10 ng/ml) or TNF-α (10 ng/ml) for 6 h. a–d The transcriptional expression of MMP1, MMP3, MMP13, and ADAMTS4 was evaluated by real time-PCR. Relative fold-change values were calculated in comparison with the vehicle control that was set to 1 (n = 3)

Back to article page

Journal of Orthopaedic Surgery and Research

ISSN: 1749-799X